A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae.
نویسندگان
چکیده
A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different non-radioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.
منابع مشابه
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ورودعنوان ژورنال:
- Leprosy review
دوره 72 4 شماره
صفحات -
تاریخ انتشار 2001